Clinical intelligence & editorial.

PLOS

Development of shelf-stable reagents and assay kits for bioluminescence applications using the capillary-assisted vitrification platform stabilization technology

Luminescence is a powerful method for detecting trace analytes and monitoring biological processes. However, most bioluminescence reagents, including luciferase and its substrates, are sensitive to temperature, limiting their usable shelf lives, and resulting in inconsistent performance. Enhancing the stability of these reagents could reduce signal variability between experiments, simplify workflows, and address cold chain storage issues. In this study, we demonstrate the application of the plat...

Reagents

Shari Radford, Yolanda Peris-Taverner, Michael Dibble, Kevin Corn, Tian Zhu, Shannon Martello, McKenzie Mayeaux, Amanda Ladd, Sankar Renu, Tejasvi Chunduri, Aniket Jadhav, Melanie Dart, Marjan Rafat, Mary Shank-Retzlaff, Laura L. Bronsart• November 5, 2026

PLOS

Microscopic risk assessment of highway weaving sections based on large-scale UAV trajectory data: What kinematic and interaction measures each detect

Microscopic risk assessment of highway weaving sections relies largely on single-vehicle kinematic indicators, yet what such indicators actually detect is rarely tested against a measure of vehicle-to-vehicle interaction. This study analyses 12,256 complete vehicle trajectories collected by UAV at seven highway weaving sections, and evaluates every trajectory along two independent dimensions: a kinematics-based composite indicator combining longitudinal acceleration, lateral acceleration and lon...

Microscopes

Zhenghua Huang, Zhibing Yuan, Mengyuan Wang, Peng Fu• November 5, 2026

PLOS

Unveiling the functional landscape of A-to-I RNA editing associated with Marek’s disease in chickens

Genetic improvement of disease resistance against viral infections represents a longstanding and formidable challenge in animal breeding. The antiviral innate immune response is highly regulated at the RNA level, especially through RNA modifications. Adenosine-to-Inosine (A-to-I) RNA editing modifies the mRNA sequence. Growing evidence shows that this type of RNA editing plays a key role in mediating cellular responses to viral infections and disease resistance. However, the role of RNA editing...

Aed

Qunhao Niu, Yi Ding, Longbin Yang, John R. Dunn, Huanmin Zhang, Keji Zhao, Jiuzhou Song• November 5, 2026

PLOS

Differential changes in cervical proprioception and motor imagery following structured imagery training in chronic neck pain

Background: Chronic neck pain (CNP) is associated with altered sensorimotor function, including impairments in cervical proprioception and changes in body-related perception. While implicit motor imagery (IMI)–based interventions have shown clinical benefits, their effects on cervical proprioceptive performance and motor imagery-related outcomes in individuals with CNP remain unclear. Objective: To investigate the effects of lateralization and IMI training on cervical proprioception (CP), neck a...

Pain

Aysegul Bostan, Aysenur Akan, Ozlem Ulger• November 5, 2026

PLOS

Supporting engagement, behavior change, and equitable self-management in menstrual and menopausal health: A multi-agent digital-health framework

Menstrual-cycle–related conditions and, later, menopausal symptoms are highly prevalent across the female life course and impose a large, under-recognized burden on quality of life and work, yet digital tools to support self-management are fragmented, hard to sustain, and unevenly reached. We propose HERA (Health Engagement and Reflective Agent architecture), a conceptual, hypothesis-generating framework for supporting engagement, behavior change, and equitable self-management in menstrual and m...

Menopause

Koichiro Yuji, Wakako Yuji, Maika Nariai, Yuki Enomoto, Osamu Wada-Hiraike• November 5, 2026

PLOS

Effectiveness of nurse-led hypertension interventions on retention, adherence, and blood pressure control among adults in low- and middle-income countries: A systematic review

Background: Hypertension affects more than 1.28 billion adults globally and remains the leading modifiable risk factor for cardiovascular disease, stroke, renal failure, and premature death. This burden is inconsistently distributed across Low- and Middle-Income Countries (LMICs), where nearly three-quarters of adults live with hypertension. This gap is largely driven by poor adherence and low retention. This systematic review aims to assess the effectiveness of nurse-led interventions on retent...

Blood Pressure

Tagese Yakob, Charuai Suwanbamrung, Cua Ngoc Le, Abebe Sorsa Badacho, Udomsak Saengow• November 5, 2026

PLOS

Modeling menstrual cycle phases from urinary hormone trajectories using an autoregressive hidden Markov model

The recent availability of at-home menstrual cycle tracking technology has created opportunities for personalized assessment of reproductive health, alongside improved characterization of hormone patterns in women with and without reproductive disorders such as polyendocrine metabolic ovarian syndrome (PMOS), which affects approximately 10% of reproductive-age women. In this study, we leverage self-tracked urinary hormone data to develop an autoregressive Hidden Markov model (arHMM) that maps cy...

Hormone

Theresa M. Stujenske, Thomas P. Bouchard, Alexis Troy, Sara Kelemen, Brianna Folino, Tara Wills, Lauren A. Sugden• November 5, 2026

PLOS

Retraction: An effective hotspot mitigation system for Wireless Sensor Networks using hybridized prairie dog with Genetic Algorithm

The PLOS One Editors retract this article [ 1 ] due to concerns about potential manipulation of the publication process. These concerns call into question the validity and provenance of the reported results. We regret that the issues were not identified prior to the article’s publication. Additionally, the following concerns were noted: Reference 3 was retracted after [ 1 ] was submitted and before [ 1 ] was published. References 24 and 28 were retracted after [ 1 ] was published. The...

Genetics

The PLOS One Editors• November 5, 2026

PLOS

PIP5K isoforms and phosphoinositide molecular species in EGF-stimulated PIP3 synthesis: A multi-method approach using enzyme genetic suppression and chemical inhibition in MCF10A cells

The membrane phospholipid PI(4,5)P2 has structural functions and is a substrate for PLC and PI3K enzymes to generate Ins(1,4,5)P3, DAG and PI(3,4,5)P3 signals. PIP5Ks, that 5-phosphorylate PI4P, are the major source of PI(4,5)P2 in mammalian cells. Current thinking suggests distinct pools of PI(4,5)P2 may be differentially generated by PIP5Kα, β and γ isoforms and involved in different physiological functions. Phosphoinositides are comprised of families of acyl-chain variants but are uniquely en...

Genetics

Iosif Alexandrou, Vikki Flemington, Martin E. Swarbrick, Sabina Cosulich, Phillip T. Hawkins, Len R. Stephens• November 5, 2026

PLOS

Unveiling Molecular Basis of Terminalia chebula in Modulating the AGE-RAGE-PRKCB Axis in Diabetic Nephropathy: Integrative Network Pharmacology and Computational Study

AbstractDiabetic nephropathy (DN), a progressive microvascular complication of diabetes, remains a principal cause of renal failure worldwide, yet its multifaceted molecular complexity continues to challenge effective therapeutic intervention. In this study, an integrative network pharmacology and computational modeling framework was employed to unravel the mechanistic basis of Terminalia chebula in mitigating DN pathology. A total of 357 phytoconstituents were retrieved from the IMPPAT 2.0 and...

Diabetes

S. M. Riaduzzaman Niaz, Sumaia Jannat, Sheikh Abdullah Al Ashik, Md. Khairul Islam• November 5, 2026

PLOS

Genotype-derived interpretive categories of drug response in 908 pharmacogenomic reports from Chinese patients with type 2 diabetes: A retrospective single-centre study

Pharmacogenomic testing is increasingly ordered in routine diabetes care, but the reports it generates are heterogeneous and are not in a form that can be analysed systematically. We examined how genotype is translated into interpretive categories of drug response in a real-world Chinese cohort, and how consistently that translation is applied. We analysed 908 de-identified pharmacogenomic reports issued for patients with type 2 diabetes at a single centre between July 2022 and April 2024. An in...

Diabetes

Ye Liu, Chun-xiao Yu, Li Lu• November 5, 2026

PLOS

Depressive symptoms among people living with HIV in Northern Tanzania: The role of family history, social support, and maladaptive coping

Background: Depression is a common mental disorder among people living with HIV (PLWH) and is driven by both neuroimmunological and psychosocial pathways. In turn, depressive symptoms worsen HIV outcomes through reduced adherence, risk behaviors, and impaired immune function. This study aimed to determine the prevalence of depressive symptoms and identify associated factors among PLWH attending health facilities in northern Tanzania. Methodology: We conducted a cross-sectional study (September–O...

Depression

Neema Allen Ng’unda, Florian Emanuel Ghaimo, Frank Kiwango, Samuel Chacha, Blandina Theophil Mmbaga, Dorothy Mushi, Ester Steven Mzilangwe• November 5, 2026